Catalog Number - 68508-15P-3M

Cogent RP C8™ Wide Pore HPLC Columns are designed for reversed-phase separations of larger molecules that require enhanced pore accessibility and efficient mass transfer. The stationary phase consists of a traditional octyl (C8) bonded ligand attached to ultra-high purity silica and is fully double end capped to minimize secondary interactions while providing reproducible retention, excellent peak shape, and long column life. The 300 Å wide pore structure allows proteins, peptides, polysaccharides, and other higher molecular weight compounds to access more of the bonded surface area, making this phase particularly suitable for biomolecule analyses and challenging large-molecule separations.

68508-15P-3M is packed with 5 µm RP C8™ Wide Pore particles featuring a 300 Å pore size in a 4.6 mm ID x 150 mm length format. The 150 mm column length provides high resolving power and increased peak capacity for complex separations while maintaining compatibility with conventional analytical HPLC systems. The wide pore architecture improves accessibility and mass transfer for proteins, peptides, polysaccharides, and long-chain fatty acids that may be partially excluded from conventional 100 Å media. The RP C8™ chemistry provides lower hydrophobic retention than comparable C18 phases while maintaining excellent selectivity for hydrophobic and moderately non-polar compounds.

This column is frequently selected for the analysis of macromolecules using HPLC with UV detection and is also suitable for LC-MS applications when used with appropriate flow splitting. The 4.6 mm ID format operates efficiently on standard analytical HPLC systems and typically performs best at flow rates of approximately 1.0-2.0 mL/min. The combination of wide pore accessibility, analytical robustness, and increased resolving power makes this column well suited for pharmaceutical, biopharmaceutical, and research applications.

Key Benefits

• RP C8™ Wide Pore stationary phase

• Octyl (C8) bonded phase

• Fully double end capped

• Ultra-high purity silica support

• 300 Å wide pore design

• Ideal for proteins, peptides, and polysaccharides

• High resolving power

• Lower retention than C18 phases

• Low backpressure with 5 µm particles

• Compatible with UV and LC-MS detection

Typical Applications

• Reversed-phase HPLC

• Protein analysis

• Peptide analysis

• Polysaccharide analysis

• Biomolecule separations

• Long-chain fatty acid analysis

• Pharmaceutical analysis

• Biopharmaceutical analysis

• Method development

• High-resolution analytical separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C8™ Wide Pore

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 4.6 mm ID x 150 mm

• Recommended Flow Rate: 1.0-2.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Designed for large molecule reversed-phase separations

• 300 Å pores enhance accessibility for proteins, peptides, polysaccharides, and other biomolecules

• Provides lower retention than comparable C18 columns

• Suitable for hydrophobic and moderately non-polar compounds

• Excellent choice for protein, peptide, polysaccharide, and long-chain fatty acid analyses

• 150 mm length provides high resolving power and increased peak capacity

• 5 µm particles provide low backpressure and broad instrument compatibility

• Compatible with standard HPLC systems operating at conventional analytical flow rates

• Frequently selected for UV-based biomolecule analysis and LC-MS applications using flow splitting

• Manufactured using ultra-high purity silica and fully double end capped bonding technology